12/13/2022 0 Comments Hdyroxy photolinker![]() These high affinity and high specificity protein-binding molecules or ligands are referred to as capture agents. Such devices may be comprised of many different protein-binding molecules arrayed on a suitable surface in a defined pattern, each capable of recognizing its target protein with high affinity and high specificity. One approach is to construct protein-detecting arrays (Kodadek, 2001 Kodadek, 2002), akin to the DNA microarrays used widely in genomics research. ![]() There is great interest in the development of techniques with which to monitor the levels, post-translational modification states and activities of large numbers of proteins simultaneously. MECAs will be of great utility in the construction of medical diagnostic devices. Such a high affinity synthetic capture agent is referred to as a mixed element capture agent (MECA). More particularly, it concerns methods and compositions for obtaining a high affinity synthetic capture agent for a molecular or biomolecular target by co-immobilizing at least two low-to-moderate affinity ligands on a suitable surface. The present invention relates generally to the fields of chemistry, molecular biology, and diagnostics. The government has certain rights in the invention. NO1-HV-28185 awarded by the National Institutes of Health, and grant number I-1299 awarded by the Welch Foundation. This invention was made with government support under grant numbers R21CA932701 and R21CA093287 awarded by the National Cancer Institute, contract No. 9, 2003, which is incorporated herein by reference in its entirety. From these results, we conclude that the PMB-PL surface is a suitable platform to regulate cell adhesion and detachment via photochemical reaction.This application claims priority to U.S. Moreover, the extracellular expression levels of transmembrane proteins on cells detached by photoirradiation showed no significant difference with those seen on normal cells. The proliferation rate and morphological changes of cells were as the same as those of cells detached by conventional enzymatic digestion. The functionalities of the cells detached by photoreaction were evaluated. Additionally, when the polymer surface was re-used after the first photoirradiation, cells did not adhere to it, and low detachment was observed. The PL groups at the surface provide adhesion points for cells, as evidenced by the fact that after photoreaction of PL groups by photoirradiation, the number of adhered cells on the surface considerably decreased. ![]() The efficiency of cell detachment obtained was approximately 50% of the initial number of adhered cells. The PMB-PL surface allowed successful control of living cells adhesion with photoreactivity. On this prepared surface, the photoinduced control of human epidermoid carcinoma cancer cells (A431 cells) and primary murine embryonic fibroblast (PMEF) cells was examined. The surface was prepared using the amphiphilic and water-insoluble substance poly(2-methacryloyloxyethyl phosphorylcholine- co- n-butyl methacrylate) (PMB) bearing 4-butyric acid (PL) groups in its side chain (PMB-PL). Here, we describe dynamic control of cell adhesion and detachment at a photoreactive and cytocompatible phospholipid polymer surface. Regulating the detachment of adhered living cells from a surface is a key technological requirement to obtain specific cells in the field of cellular engineering. ![]()
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